Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues. |
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Authors: | Franziska Miething Sandra Hering B?rbel Hanschke Jan Dressler |
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Affiliation: | Faculty of Medicine, Institute of Legal Medicine, University of Dresden, Dresden, Germany. |
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Abstract: | Samples of different tissues were preserved in seven fixatives for periods of time extending from 1 to 336 days, to determine which fixatives reduce the time-dependent degradation of DNA and preserve the histological structure. To achieve these results, three PCR systems were used: FGA and TC11 (both for nuclear DNA) and HV1 for mitochondrial DNA (mt-DNA). For long-term storage in combination with amplification of nuclear and mt-DNA, consistent results were obtained in Carnoy's solution and glutaraldehyde. Variable results were observed for buffered formalin; an mt-DNA product could be detected even after 3 months of fixation. In regard to comparison of the different tissues, the quantities recovered from skeletal muscles and kidneys were higher than from other tissues. |
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