苜蓿悬浮细胞对盐胁迫的反应和适应

苜蓿悬浮细胞能够适应200mmol/L NaCl及其以下盐浓度的胁迫,适应细胞中游离脯氨酸、还原糖和Na~ 积累增加。400mmol/LNaCl对细胞生长明显抑制。细胞对盐胁迫的反应和适应中PM-ATPase和TM-ATPase起到重要作用,在适应细胞中两者的活力都明显增加。PM-ATPase活力的增加可受CHX的明显抑制。

REACTION AND ADAPTATION OF SUSPENSION CULTURE CELLS OF ALFALFA ON SALINITY

Suspension cultural cells of alfalfa can be adaptation to grown in levels of 200 millimolar NaCl. The 200 milimolar NaCl adaptation cells are increased in both growth rate and respiration rate. But the 400 millimolar NaCl adaptation cells are inhabitated obviously in the both rates. There are enchanced the higher concentration of Na , proline and water soluble reducing-sugars in adaptation cells. It is proposed that Na , proline and water soluble reducing-sugars accumulation are the main component of salt adaptation of the suspension cultural cells. Both plasma membrane ATPase and tonoplast membrane ATPase activity of the adaptation cells are increased. Both the enzymes play moreimportant roles to the intracellular com-partmentation of ions in salt adaptation cells (Reinhold 1989, Binzel 1989). The PM-ATPase plays a leading role in lower NaCl concetration (200 milimolar) adaptation cells, and the TM-ATPase plays a leading role in higher NaCl levels (400 millimolar) adaptation cells. When adding a salt stress, the NaCl adaptation cells of alfalfa may start the osmoregulation controlling system more quickly than the NaCl unadaptation cells. During the salt stress, the increase of PM-ATPase activity can be inhabited with CHX. It tell us that there may be synthesis of new pro-tiens in the cells during that time.