Assay for cellobiose dehydrogenase in the presence of laccase |
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| Authors: | G Henriksson V Polk K-EL Eriksson |
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| Institution: | (1) Department of Biochemistry and Molecular Biology, Center for Biological Resource Recovery, University of Georgia, Athens, GA 30602-7229, USA |
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| Abstract: | The currently used assay for cellobiose dehydrogenase (CDH), an enzyme produced by many wood degrading fungi, lacks specificity and can give false results. The presence of laccase interferes with the standard assay. We have developed an assay for CDH that is insensitive to the presence of both laccase and other phenoloxidases. The method is based on the decrease of reducing end groups in lactose determined by the DNS method. Ferricyanide is present as electron acceptor. Advantages and drawbacks of CDH assay methods are discussed |
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