首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Intestinal tube formation in Caenorhabditis elegans requires vang-1 and egl-15 signaling
Authors:Michael Hoffmann  Christoph Segbert  Gisela Helbig
Institution:a Institute of Molecular and Cellular Anatomy (MOCA), RWTH Aachen University, D-52074 Aachen, Germany
b Institute for Genetics, Heinrich-Heine-University Düsseldorf, D-40225 Düsseldorf, Germany
c Department of General Pediatrics, Heinrich-Heine-University, D-40225 Düsseldorf, Germany
Abstract:Understanding how epithelial organs form during morphogenesis is a major problem in developmental biology. In the present paper, we provide a detailed analysis of vang-1, the only homolog of the planar cell polarity protein Strabismus/Van Gogh in Caenorhabditis elegans. We demonstrate that during organogenesis of the intestine, (i) VANG-1 specifically interacts with PDZ 2 domain of DLG-1 (Discs large) and becomes phosphorylated by the kinase domain of the FGF-like receptor tyrosine kinase EGL-15; (ii) VANG-1 is predominantly restrained to the cell cortex but relocates to the apical junction; and (iii) in vang-1 embryos epithelial cells of the intestine are not correctly arranged along the anterior-posterior axis. To investigate what determines the disposition of the VANG-1 protein, either truncated protein forms were expressed in the intestine or RNAi was used to remove the functions of gene products previously shown to be involved in apical junction formation. Removal of the VANG-1 PDZ binding motif “− ESAV” and depletion of dlg-1 or let-413 gene functions interferes with the localization of VANG-1. In addition, egl-15 embryos show a premature relocation of VANG-1 to the apical junction, causing defects that resemble those observed in mutant vang-1 embryos and after intestine-specific overexpression of full-length vang-1. Finally, the localization of VANG-1 depends on DSH-2, a homolog of the planar cell polarity protein Dishevelled and depletion phenocopies vang-1 and egl-15 phenotypes in the embryonic intestine.
Keywords:CeAJ  Caenorhabditis elegans apical junction  DAC  DLG-1-AJM-1 complex  mab  monoclonal antibody  PCP  planar cell polarity  PDZ (PSD95  Discs large  ZO-1)  RNAi  RNA-mediated interference  RT  room temperature  Y2HA  yeast two-hybrid analysis  WT  wild type
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号