Genetic dissection of chromosome substitution lines of cotton to discover novel Gossypium barbadense L. alleles for improvement of agronomic traits |
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Authors: | Sukumar Saha Jixiang Wu Johnie N Jenkins Jack C McCarty Russell Hayes David M Stelly |
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Institution: | (1) Crop Science Research Laboratory, USDA-ARS, 810 Highway 12 East, Mississippi State, MS 39762-5367, USA;(2) Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS 39762, USA;(3) Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA;(4) Present address: Plant Science Department, South Dakota University, Brookings, SD 57007, USA |
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Abstract: | We recently released a set of 17 chromosome substitution (CS-B) lines (2n = 52) that contain Gossypium barbadense L. doubled-haploid line ‘3-79’ germplasm systematically introgressed into the Upland inbred ‘TM-1’ of G. hirsutum (L.). TM-1 yields much more than 3-79, but cotton from the latter has superior fiber properties. To explore the use of these
quasi-isogenic lines in studying gene interactions, we created a partial diallel among six CS-B lines and the inbred TM-1,
and characterized their descendents for lint percentage, boll weight, seedcotton yield and lint yield across four environments.
Phenotypic data on the traits were analyzed according to the ADAA genetic model to detect significant additive, dominance,
and additive-by-additive epistasis effects at the chromosome and chromosome-by-chromosome levels of CS-B lines. For example,
line 3-79 had the lowest boll weight, seedcotton yield and lint yield, but CS-B22Lo homozygous dominance genetic effects on
seedcotton and lint yield were nearly four times those of TM-1, and its hybrids with TM-1 had the highest additive-by-additive
epistatic effects on seedcotton and lint yield. CS-B14sh, 17, 22Lo and 25 produced positive homozygous dominance effects on
lint yield, whereas doubly heterozygous combinations of CS-B14sh with CS-B17, 22Lo and 25 produced negative dominance effects,
suggesting that epistatic effects between genes in these chromosomes strongly affect lint yield. The results underscore the
opportunities to systematically identify genomic regions harboring genes that impart agronomically significant effects via
epistatic interactions. The chromosome-by-chromosome approach significantly complements other strategies to detect and quantify
epistatic interaction effects, and the quasi-isogenic nature of families and lines from CS-B intermatings will facilitate
high-resolution localization, development of markers for selection and map-assisted identification of genes involved in strong
epistatic effects. |
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