Nitric oxide is involved in heat-induced HSP70 accumulation |
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| Authors: | I. Yu. Malyshev E. B. Manukhina V. D. Mikoyan L. N. Kubrina A. F. Vanin |
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| Affiliation: | aInstitute of General Pathology and Pathophysiology, Baltijskaya 8, Moscow 125315, Russian Federation;bInstitute of Chemical Physics, Kosygin Str. 4, Moscow 117977, Russian Federation |
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| Abstract: | Heat shock potentiated the nitric oxide production (EPR assay) in the liver, kidney, heart, spleen, intestine, and brain. The heat shock-induced sharp transient increase in the rate of nitric oxide production preceded the accumulation of heat shock proteins (HSP70) (Western blot analysis) as measured in the heart and liver. In all organs the nitric oxide formation was completely blocked by the NO-synthase inhibitor (L-NNA). L-NNA also markedly attenuated the heat shock-induced accumulation of HSP70. The results suggests that nitric oxide is involved in the heat shock-induced activation of HSP70 synthesis. |
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| Keywords: | Heat shock EPR Western blot analysis Nitric oxide Stress proteinBP, blood pressure DETC, Fe2+-diethyldithiocar-bamate EPR, electron paramagnetic resonance HS, heat shock HSP70 heat shock proteins with molecular weight 70 kDa L-NNA, MNIC, mononitrosyl-iron complex NO, nitric oxide cNOS, constituitive NO-synthase iNOS, inducible NO-synthase |
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