Essentiality of a Newly Identified Carbohydrate-Binding Module for the Function of CelB (BH0603) from the Alkaliphilic Bacterium Bacillus halodurans

CelB (BH0603) from Bacillus halodurans is a modular glycoside hydrolase with a family 5 catalytic module, an immunoglobulin-like module, and module PfamB of unknown function. The recombinant PfamB module bound to Avicel and was essential for CelB hydrolytic function. We propose that module PfamB be designated a new carbohydrate-binding module.