EFEMP2 Mediates GALNT14-Dependent Breast Cancer Cell Invasion |
| |
Authors: | Tao Zuo Jinshuai Shan Yang Liu Rong Xie Xiaochun Yu Chen Wu |
| |
Institution: | 2. Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope, 1500 E. Duarte Rd, Duarte, California, 91010, USA |
| |
Abstract: | N-Acetylgalactosaminyltransferase-14 (GALNT14) is a member of acetylgalactosaminyltransferases family. We have shown that GALNT14 could promote breast cancer cell invasion. However, the underlying molecular mechanism is unclear. Here, using yeast two hybrid, we find that EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) interacts with GALNT14. Both in vitro and in vivo binding assays show that EFEMP2 is associated with GALNT14. Moreover, we find that GALNT14 mediates glycosylation of EFEMP2. EFEMP2 significantly increased the invasion ability of breast cancer cells including MCF-7 and MBA-MD-231 cells, and this phenomenon is suppressed by knockdown expression of GALNT14. In addition, the GALNT14-dependent O-glycosylation of EFEMP-2 regulates the stability of EFEMP-2 protein in breast cancer cells. Taken together, our results demonstrate a novel molecular mechanism underlying breast cancer invasion. |
| |
Keywords: | Address all correspondence to: Xiaochun Yu or Chen Wu College of Life Sciences Hebei University Baoding 071002 Hebei P R China |
本文献已被 ScienceDirect 等数据库收录! |
|