| 牛脑液泡型质子泵70kD和33kD亚基基因的表达 |
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| 引用本文： | 张瑛,彭生斌,D.K.Stone,谢小松. 牛脑液泡型质子泵70kD和33kD亚基基因的表达[J]. 生物化学与生物物理进展, 1994, 21(4): 342-347 |
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| 作者姓名： | 张瑛 彭生斌 D.K.Stone 谢小松 |
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| 作者单位： | 中国科学院生物物理研究所;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A.;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A.;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A. |
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| 基金项目： | 美国NIH(DK RO1-33627)基金资助. |
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| 摘 要： | 已分离了编码牛脑液泡型质子泵的70kD亚基的cDNA,利用聚合酶链反应(PCR)扩增了70kD亚基的编码片段,同时直接从牛脑cDNA库中得到了33kD亚基的编码片段.分别将相应片段连接到PET载体上完成70kD和33kD亚基基因在大肠杆菌中的表达.SDS聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明70kD和33kD亚基基因均得到明显表达.
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| 关 键 词： | 质子泵 70kD和33kD亚基 基因表达 |
| 收稿时间： | 1993-06-25 |
| 修稿时间： | 1993-11-24 |
Expression of the Genes of the 70kD and 33kD Subunits of the Bovine Brain Vacuolar Proton Pump in E. coli |
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| Zhang Ying,Peng Shengbin,Stone D. K. and Xie Xiaosong. Expression of the Genes of the 70kD and 33kD Subunits of the Bovine Brain Vacuolar Proton Pump in E. coli[J]. Progress In Biochemistry and Biophysics, 1994, 21(4): 342-347 |
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| Authors: | Zhang Ying Peng Shengbin Stone D. K. Xie Xiaosong |
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| Affiliation: | Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A.;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A.;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A.;Deparrment of Internal Medicine, Division of Molecular Transport, the University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, U. S. A. |
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| Abstract: | The genes of the 70kD and 33kD subunits of the bovine brain vacuolar proton pump have been successfully expressed in E. Coli. The fragment of the gene of 70kD subunit was obtained by polymerase chain reaction (PCR) from an isolated cDNA encoding the 70kD subunit of the bovine brain vacuolar proton pump. The fragment of the gene of 33kD subunit was also obtained by PCR from the bovine brain cDNA library. Two clones encoding 70kD and 33kD subunits were individually obtained by directly joining the PCR products into PET vector. Sodium dodecyl sulfate (SDS)-poly-acrylamide gel electrophoresis (PAGE)and Western blot analysis of cultured transformants demonstrated high expression. |
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| Keywords: | vacuolar proton pump 70kD and 33kD subunits expression of genes |
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