Inositol 1,4,5-trisphosphate 3-kinase A is a novel microtubule-associated protein: PKA-dependent phosphoregulation of microtubule binding affinity |
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Authors: | Lee Dongmin Lee Hyun Woo Hong Soontaek Choi Byung-Il Kim Hyun-Wook Han Seung Baek Kim Il Hwan Bae Jin Young Bae Yong Chul Rhyu Im Joo Sun Woong Kim Hyun |
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Affiliation: | Department of Anatomy, College of Medicine, Korea University, Brain Korea 21, Seoul 136-705, Korea. |
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Abstract: | Inositol 1,4,5-trisphosphate 3-kinase A (IP(3)K-A) is a brain specific and F-actin-binding protein. We recently demonstrated that IP(3)K-A modulates a structural reorganization of dendritic spines through F-actin remodeling, which is required for synaptic plasticity and memory formation in brain. However, detailed functions of IP(3)K-A and its regulatory mechanisms involved in the neuronal cytoskeletal dynamics still remain unknown. In the present study, we identified tubulin as a candidate of IP(3)K-A-binding protein through proteomic screening. By various in vitro and in vivo approaches, we demonstrated that IP(3)K-A was a novel microtubule-associated protein (MAP), and the N terminus of IP(3)K-A was a critical region for direct binding to tubulin in dendritic shaft of hippocampal neurons. Moreover, PKA phosphorylated Ser-119 within IP(3)K-A, leading to a significant reduction of microtubule binding affinity. These results suggest that PKA-dependent phosphorylation and microtubule binding of IP(3)K-A are involved in its regulatory mechanism for activity-dependent neuronal events such as local calcium signaling and its synaptic targeting. |
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Keywords: | Cytoskeleton Microtubules Phosphorylation Protein Kinase A (PKA) Tubulin IP3K-A ITPKA |
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