Oligomeric organization of recombinant human neurotrophins expressed in <Emphasis Type="Italic">Escherichia coli</Emphasis> cells |
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| Authors: | D R Safina L M Rafieva A V Koval’ E E Shkurina V G Dmitrieva N M Raevskaya E V Gasanov I V Demidyuk S V Kostrov |
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| Institution: | (1) Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 2, Moscow, 123186, Russia |
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| Abstract: | Genes of human neurotrophins NGF, BDNF, NT-3 were cloned, and the corresponding proteins and their fragments were expressed in Escherichia coli BL-21 (DE3λ) cells. Their intracellular localization was determined. The conditions for isolation and purification of the target recombinant proteins and for folding of BDNF and NT-3 precursors were selected. The recombinant proprecursors of human neurotrophines have been shown to possess complex oligomeric structure. |
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| Keywords: | prodependent folding prosequence recombinant human proneurotrophines |
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