Co-localization of a glucose transporter and the insulin receptor in microsomes of insulin-treated rat adipocytes |
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Authors: | O Ezaki N Bono H Itakura M Kasahara |
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Institution: | Division of Nutrition and Disease, National Institute of Nutrition, Tokyo, Japan. |
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Abstract: | Microsomal vesicles prepared from rat adipocytes were immuno-adsorbed to formaldehyde-fixed Staphylococcus aureus cells (Pansorbin) coated with anti-human-erythrocyte-glucose-transporter IgG. More than 75% of the glucose transporter detected was precipitated. The glucose transporter was about 10-fold enriched by the adsorption procedure. On insulin treatment, the insulin receptor in plasma membranes was internalized and the receptor in the microsome fraction increased 5-fold. Thirty-five % of the insulin receptor in the microsome fraction was recovered with the glucose-transporter-containing vesicles. These observations indicate that on insulin treatment a considerable portion of the microsome vesicles containing the insulin receptor fuses or becomes tightly associated with ones containing the glucose transporter. |
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