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Control of in vitro growth of viviparous embryo mutants of maize by abscisic acid
Authors:C S Robichaud  J Wong  I M Sussex
Abstract:The plant hormone abscisic acid (ABA) is believed to play a role in the onset of developmental arrest in seeds. Embryos of the viviparous mutants of Zea mays do not undergo arrest but germinate directly on the ear. This study investigates the possibility that the mutants vp1, vp5, vp7, vp8, and vp9 are defective in some aspect of ABA action. Mutant and wild type embryos were removed from developing seeds at 18, 21, and 24 days after pollination and cultured aseptically on media containing a range of ABA concentrations. Seedlings were harvested after seven days when lengths and fresh and dry weights were recorded. The results indicate that these five viviparous mutants differ in their response to ABA. Two mutants, vp5 and vp8, exhibit the same sensitivity to growth inhibition by ABA as wild type. The remaining three mutants, however, manifest a range of decreased sensitivities with vp1 being the least sensitive, followed by vp7 and vp9.
Keywords:abscisic acid  embryo culture  vivipary  Zea mays
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