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Liver microsomal bilirubin UDP-glucuronyltransferase disturbances in bile duct ligated rats
Authors:L Bengochea  G Ouvi?a  A Lemberg
Institution:1. L.I. Medved''s Research Center of Preventive Toxicology, Food and Chemical Safety, Kyiv, Ukraine;2. Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, Basel 4056, Switzerland;3. Swiss Centre for Human Applied Toxicology, University of Basel, Missionsstrasse 64, Basel 4055, Switzerland;4. Computational Pharmacy, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, Basel 4056, Switzerland;1. Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran;2. Department of Anatomy and Embryology, School of Medicine, Bam University of Medical Sciences, Bam, Iran;3. Department of Medical Genetics, School of Medicine, Bam University of Medical Sciences, Bam, Iran;1. Bioprinting Laboratories Inc., 12200 Ford Road, Dallas, TX 75234, United States of America;2. Department of Biomedical Engineering, University of North Texas, 3940 North Elm Street, Denton, TX 76207, United States of America
Abstract:The activity of bilirubin UDP-Glucuronyltransferase was determined in microsomes from normal and bile duct ligated rats. It was measured after 2 and 8 days following bile duct ligation and compared with normal rats. A decrease of 33% in the total enzyme activity was observed on day 2; a fall of 70% was founded on day 8. Bilirubin diglucuronide represented approximately 20% of total conjugates in both groups of cholestatic rats, as compared with 65% found in normals. It was concluded that bilirubin microsomal conjugating capacity is markedly altered during cholestasis. This can be attributed to microsomal membrane damage produced by stagnant bile.
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