新型冠状病毒相关TMPRSS2蛋白结构特征和抗原表位分析

采用生物信息学方法分析预测新型冠状病毒(Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)跨膜蛋白酶丝氨酸2(transmembrane protease serine 2,TMPRSS2)的理化特性、结构特征和抗原表位,为抗SARS-CoV-2药物研...

Protein Structural Characteristics and Antigen Epitopes Analysis of TMPRSS2 Correlated with SARS-CoV-2

This study adopted bioinformatics methods to analyzes and prognoses the physicochemical properties, structural characteristics, and antigen epitopes of transmembrane protease serine 2 (TMPRSS2), to provide references for medicine research and development against SARS-CoV-2. The physicochemical properties of TMPRSS2 were analyzed and predicted adopting ProtParam and ProtScale; the structural and functional structures of TMPRSS2 protease were analyzed and predicted using servers of COILS Server, SignalP, TMPred, TargetP Server, NetPhos Server, NetNGlyc Server; the high-level structure of TMPRSS2 protease were analyzed and predicted by SOPMA, Pfam, SWISS-MODEL; the B cell and T cell epitopes of TMPRSS2 protease were analyzed and predicted by IEBD. TMPRSS2 comprised 492 amino acids, among them serine accounts for the highest proportion, with high hydrophilic, containing 10 transmembrane helix domains, 4 phosphorylation sites, and 3 glycosylation modification sites; random coils occupied dominant position in secondary structure, while tertiary structure could be constructed with homologous of known 5ce.1.1.A (SMTL ID) model, and existed 13 potential B cell epitopes, and 12 potentially high scored T cell epitopes.