| 高通量自动化微生物微液滴进化培养与筛选技术及其装备化 |
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| 引用本文： | 郭肖杰,王立言,张翀,邢新会. 高通量自动化微生物微液滴进化培养与筛选技术及其装备化[J]. 生物工程学报, 2021, 37(3): 991-1003 |
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| 作者姓名： | 郭肖杰 王立言 张翀 邢新会 |
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| 作者单位： | 1 清华大学 化学工程学系 生物化工研究所，北京 100084;2 工业生物催化教育部重点实验室，北京 100084;3 清华大学 无锡应用技术研究院 生物育种中心，江苏 无锡 214000;1 清华大学 化学工程学系 生物化工研究所，北京 100084;2 工业生物催化教育部重点实验室，北京 100084;4 清华大学 合成与系统生物学中心，北京100084;1 清华大学 化学工程学系 生物化工研究所，北京 100084;2 工业生物催化教育部重点实验室，北京 100084;4 清华大学 合成与系统生物学中心，北京100084;5 清华大学 深圳国际研究生院生物医药与健康工程研究院 深圳湾实验室医药健康技术与工程研究所，广东 深圳 518055 |
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| 基金项目： | 国家重点研发计划 (No. 2019YFA090091)，国家自然科学基金重大仪器专项 (No.21627812) 资助。 |
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| 摘 要： | 液滴微流控由于可以快速生成大量微液滴,并实现单个液滴独立的控制,每个液滴都可以作为独立的单元进行微生物培养,因此在微生物的高通量培养方面具有独特的应用优势.然而现有研究多停留在实验室搭建和使用阶段,存在操作要求高、影响因素多、缺乏自动化集成技术等关键问题,制约了液滴微流控技术在微生物研究中的应用.文中以解决液滴微流控技...
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| 关 键 词： | 液滴微流控 微生物培养 高通量 微液滴 微生物进化 筛选 |
| 收稿时间： | 2020-10-18 |
Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening |
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| Xiaojie Guo,Liyan Wang,Chong Zhang,Xin-Hui Xing. Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening[J]. Chinese journal of biotechnology, 2021, 37(3): 991-1003 |
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| Authors: | Xiaojie Guo Liyan Wang Chong Zhang Xin-Hui Xing |
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| Affiliation: | 1 Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2 Key Laboratory for Industrial Biocatalysis of the Ministry of Education, Beijing 100084, China;3 Biobreeding Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi 214000, Jiangsu, China;1 Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2 Key Laboratory for Industrial Biocatalysis of the Ministry of Education, Beijing 100084, China;4 Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; 1 Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2 Key Laboratory for Industrial Biocatalysis of the Ministry of Education, Beijing 100084, China;4 Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;5 Shenzhen Bay Laboratory, Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School and Institute of Biomedical Health Technology and Engineering, Tsinghua University, Shenzhen 518055, Guangdong, China |
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| Abstract: | Since microdroplets are able to be generated rapidly in large amount and each droplet can be well controlled as an independent micro-cultivator, droplet microfluidic technology can be potentially used in the culture of microorganisms, and provide the microbial culture with high throughput manner. But its application mostly stays in the laboratory-level building and using for scientific research, and the wide use of droplet microfluidics in microbial technology has been limited by the key problems that the operation for microdroplets needs high technical requirements with wide affecting factors and the difficulties in integration of automatic microdroplet instrumentation. In this study, by realizing and integrating the complicated operations of droplet generation, cultivation, detection, splitting, fusion and sorting, we design a miniaturized, fully automated and high-throughput microbial microdroplet culture system (MMC). The MMC can be widely used in microbial growth curve test, laboratory adaptive evolution, single factor and multi-level analysis of microbial culture, metabolite detection and so on, and provide a powerful instrument platform for customized microbial evolution and screening aiming at efficient strain engineering. |
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| Keywords: | droplet microfluidic microbial culture high-throughput droplet microbialevolution screening |
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