苦荞重组自交系群体籽粒黄酮含量与产量性状分析

为揭示影响苦荞籽粒黄酮含量的主要因素,获得高黄酮含量的苦荞种质,该文以"小米荞×晋荞2号"重组自交系(RILs)群体为材料,采用酶标仪检测籽粒黄酮含量,调查了百粒重、籽粒长宽比、株高、分枝数及籽粒产量等性状的变异,并探究籽粒黄酮含量与产量性状之间的相关关系,比较了不同粒色、不同粒型苦荞株系的籽粒黄酮含量的差异,基于聚类...

Variation analysis of flavonoid contents in seeds and yield traits on recombinant inbred line population of tartary buckwheat

In order to reveal the main factors affecting the flavonoids content of tartary buckwheat in seeds and select high flavonoids content lines,we determined the flavonoids content in seeds of a tartary buckwheat recombinant inbred lines(RILs)population derived from a cross of“Xiaomiqiao×Jinqiao 2”.The genetic variation of seed yield,100-seed weight,seed length to width ratio,plant height,branch number and flavonoids content in seeds were analyzed on RILs population.The relationship between flavonoids content in grains and yield traits was explored by correlation analysis.Variance analysis of flavonoids content in RILs population with different seed colors or shell types was carried out,cluster analysis for the RILs population was carried out based on the phenotypic data of investigated traits,and the excellent lines were selected by variance analysis among groups.The results were as follows:(1)The flavonoids content in seeds,100-seed weight,seed yield,and seed length to width ratio of‘Jinqiao 2’were significantly higher than those of‘Xiaomiqiao’;The flavonoids content in seeds of the RILs population ranged from 1.34%to 3.05%with the coefficient variation of 12.23%;The coefficient of variation of yield traits ranged from 9.38%to 25.17%,in which grain yield was the largest,followed by 100-seed weight,and seed length to width ratio was the smallest;The tremendous transgressive segregation for flavonoids content in seeds was observed in the population.(2)There was no significant correlation between flavonoids content in seeds and the investigated agronomic traits;Seed yield was significantly positively correlated to 100-seed weight and plant height.(3)There were no significant differences in flavonoids content among lines with different seed colors or shell types.(4)The RILs population could be divided into six groups at the euclidean distance of 17.6-20.6.Group C3 and Group C6 had the highest flavonoids content in seeds,with the average values of 2.39%and 2.35%respectively.Group C2 had fine performance in plant height,branch number,seed yield,100-seed weight,and seed length to width ratio.