兔出血症病毒2型理化特性研究

[目的]本研究旨在研究该病毒的理化特性,评价不同处理条件对RHDV2的杀灭效果.[方法]本研究拟对临床疑似RHDV2感染致死的家兔进行RT-PCR鉴定病原,并利用不同pH值、不同温度、常用兽用消毒剂、不同浓度甲醛处理RHDV2,通过PMA-RT-qPCR对病毒理化特性进行研究.[结果]经RT-PCR检测与测序分析,确诊...

Physicochemical properties of rabbit hemorrhagic disease virus type 2

[Objective]The purpose of this study was to study the physicochemical properties of the virus RHDV2 and evaluate effect of different treatment to kill the virus.[Methods]The virus was identified by RT-PCR in rabbits with clinical suspected death from RHDV2 infection.The physicochemical properties of RHDV2 were studied by PMA-RT-qPCR at different pH,different temperatures,common veterinary disinfectants and different concentrations of formaldehyde.[Results]RHDV2 infection was confirmed by RT-PCR detection and sequencing analysis.The VP60 gene sequence of this strain was 98.35%consistent with RHDV2 in GenBank(MN276176.1).PMA-RT-qPCR results show that RHDV2 was sensitive to pH,high temperature,animal disinfectant and formaldehyde.The killing rate of virus increased with pH decreasing or increasing.The virus killing rate reached 77.61%after being treated at 65℃for 30 min,and 95.10%after being treated at 83℃for 5 min.RHDV2 is sensitive to all three animal disinfectants,among which povidone iodine had the best killing effect on RHDV2,the virus killing rate reached 88.78%after 30 min.The killing rate of 0.3%formaldehyde was better than 0.2%,and after 30 min of application,the killing rate could reach 82.22%.[Conclusion]RHDV2 is sensitive to acid and base,high temperature,animal disinfectant and formaldehyde to varying degrees.