DSF-家族群体感应信号生物合成途径与调控机制研究进展

群体感应是微生物间相互交流的一种重要机制.Diffusible Signaling Factor(DSF)-家族群体感应信号分子存在于多种革兰氏阴性菌中,调控细菌的致病性和适应性.本文首先介绍DSF-家族群体感应信号的结构多样性与保守性、生物合成途径和两类调控机制.DSF家族群体感应信号属于一类长链不饱和脂肪酸,碳水化...

Research progress in the biosynthetic pathway and regulatory mechanisms of diffusible signaling factor-family quorum sensing signals

Quorum sensing (QS) is one of the most important cell-cell communication mechanisms used by bacteria. DSF-family QS signals are present in a range of Gram-negative bacterial species, regulating bacterial virulence and adaptation. In this review, the structural diversity and conservation of DSF-family QS signals, their biosynthetic pathways and two regulatory mechanisms controlling DSF biosynthesis were firstly introduced. DSF-family signals are all long-chain unsaturated fatty acids, their biosynthetic precursors include carbohydrates and branched-chain amino acids; the major biosynthetic pathway include fatty acid elongation cycle and the key enzyme RpfF with both dehydratase and thioesterase activities; two regulatory mechanisms involving protein-protein interactions were identified to control DSF biosynthesis in Xanthomonas and Burkholderia, respectively. Then, the key in planta QS signal used by Xanthomonas campestris pv. campestris during its infection of Chinese cabbage was introduced. Finally, some key scientific questions in this research area were discussed and prospected.