槲蕨属叶绿体基因组密码子偏好性分析

叶绿体基因组密码子偏好性使用模式往往影响基因表达效率,为促进药用植物叶绿体基因工程的发展,提高槲蕨药用品质,该研究以川滇槲蕨、栎叶槲蕨和槲蕨三个近缘药用植物为材料,使用CodonW、CUSP和SPSS等软件分析其叶绿体基因组编码基因密码子使用偏好性,筛选出三个物种的最优密码子.结果表明:川滇槲蕨、栎叶槲蕨和槲蕨叶绿体基...

Condon preference of chloroplast genome of Drynaria

The codon usage patterns of the chloroplast genes were related to the expression efficiency of genes. In order to improve the development of the chloroplast genetic engineering of medicinal plant and to promote the quality of medicinal plant Drynaria roosii, the optimal codons in chloroplast genome were identified through analyzing the factors shaping of codon bias of D. delavayi, D. quercifolia and D. roosii using CodonW, CUSP and SPSS softwares. The results were as follows: The effective number of codons(ENC)of the chloroplast gene ranged from 40.10 to 61, from 40.33 to 61, and from 40.15 to 61 in D. delavayi, D. quercifolia, and D. roosii, respectively, which suggested the codon bias was weak among the three species; The ENC of gene ndhE, rpl22, rpl14, rpl20, ccsA, rps4 and rpl16 showed significant difference among species, which suggested the codon bias of some genes was various in closely species; The distribution of ENC frequency ratio of most coding gene ranged from -0.1 to 0.1, suggesting the codon usage bias was produced by gene mutation in Drynaria species. In addition, 12 codons were identified as optimal codons in D. delavayi, ten in D. quercifolia, and three in D. roosii. The codons UCU, ACU, GCU, CAA, AAA and GAU were the optimal codons in D. delavayi and D. quercifolia, the codons UUA and AUU were the optimal codons in D. quercifolia and D. roosii, while did not in D. delavayi and D.roosii. Our results will be useful for the codon preference analysis of ferns and the chloroplast genetic engineering of medicinal plant.