An ordered EST catalogue and gene expression profiles of cassava (<Emphasis Type="Italic">Manihot esculenta</Emphasis>) at key growth stages |
| |
Authors: | You-Zhi Li Ying-Hua Pan Chang-Bin Sun Hai-Tao Dong Xing-Lu Luo Zhi-Qiang Wang Ji-Liang Tang Baoshan Chen |
| |
Institution: | (1) Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, College of Life Science and Technology, Guangxi University, 530004 Nanning, Guangxi, People’s Republic of China;(2) Agricultural College, Guangxi University, 530004 Nanning, Guangxi, People’s Republic of China;(3) Institute of Biotechnology, Zhejiang University, 310029 Hangzhou, Zhejiang, People’s Republic of China; |
| |
Abstract: | A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600
cDNA clones from the library were sequenced with single-pass from the 5′-terminus to establish a catalogue of expressed sequence
tags (ESTs). Assembly of the resulting EST sequences resulted in 2,878 putative unigenes. Blastn analysis showed that 62.6%
of the unigenes matched with known cassava ESTs and the rest had no ‘hits’ against the cassava database in the integrative
PlantGDB database. Blastx analysis showed that 1,715 (59.59%) of the unigenes matched with one or more GenBank protein entries
and 1,163 (40.41%) had no ‘hits’. A cDNA microarray with 2,878 unigenes was developed and used to analyze gene expression
profiling of Huanan 124 at key growth stages including seedling, formation of root system, root bulking, and starch maturity.
Array data analysis revealed that (1) the higher ratio of up-regulated ribosome-related genes was accompanied by a high ratio
of up-regulated ubiquitin, proteasome-related and protease genes in cassava roots; (2) starch formation and degradation simultaneously
occur at the early stages of root development but starch degradation is declined partially due to decrease in UDP-glucose
dehydrogenase activity with root maturity; (3) starch may also be synthesized in situ in roots; (4) starch synthesis, translocation,
and accumulation are also associated probably with signaling pathways that parallel Wnt, LAM, TCS and ErbB signaling pathways
in animals; (5) constitutive expression of stress-responsive genes may be due to the adaptation of cassava to harsh environments
during long-term evolution. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|