Measurement of Protein Degradation in Leaves of Zea mays Using [H]Acetic Anhydride and Tritiated Water |
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Authors: | Simpson E |
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Institution: | Department of Biology, Washington University, St. Louis, Missouri 63130. |
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Abstract: | The rate of protein degradation in Zea mays leaves has been estimated by using tritiated water and 3H]acetic anhydride as the labeling agents. Both methods circumvent many of the problems usually associated with measuring protein degradation in plants. The half-life of ribulose-1,5-bisphosphate carboxylase protein in second leaves of 13-day-old seedlings under continuous light was found to be 7.8 ± 0.9 days by the tritiated water technique and 6.5 ± 0.8 days by the 3H]acetic anhydride method. The half-lives determined under a 14-hour-light, 10-hour-dark photoperiod are 6.2 ± 0.8 days with tritiated water and 5.4 ± 0.4 days with 3H]acetic anhydride. Whereas the values obtained by the two methods do not differ significantly, the use of either method for the determination of protein half-life can be recommended. |
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