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Development of media for the mixotrophic/heterotrophic culture ofBrachiomonas submarina
Authors:Alexander J Tsavalos  John G Day
Institution:(1) School of Biological and Earth Sciences, Liverpool John Moores University, Byrom St., Liverpool, L3 3AF, UK;(2) Culture Collection of Algae and Protozoa, Institute of Freshwater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria, LA22 OLP, UK
Abstract:The marine algaBrachiomonas submarina var.pulsifera (Droop) CCAP 7/2A, is employed as a food organism in aquaculture; it can be cultured heterotrophically or mixotrophically. Growth rates and productivity under heterotrophic conditions were lower than those achieved under mixotrophic conditions. By reducing the osmotic potential of the medium, whilst simultaneously increasing the levels of nitrogen and phosphorus and using sodium acetate as a carbon source, a 20-fold increase in final yield was attained. This corresponded to a maximum culture of 9.02times 106 cells ml–1 and a dry weight of 2.51 g l–1.Author for correspondence
Keywords:Brachiomonas  biotechnology  heterotrophic/mixotrophic culture  aquaculture
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