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The antimicrobial peptide tachyplesin III coated alone and in combination with intraperitoneal piperacillin-tazobactam prevents ureteral stent Pseudomonas infection in a rat subcutaneous pouch model |
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| Authors: | Minardi Daniele Ghiselli Roberto Cirioni Oscar Giacometti Andrea Kamysz Wojciech Orlando Fiorenza Silvestri Carmela Parri Gianni Kamysz Elzbieta Scalise Giorgio Saba Vittorio Giovanni Muzzonigro |
| Institution: | aInstitute of Maternal and Children's Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, 60020 Ancona, Italy bDepartment of General Surgery, I.N.R.C.A. I.R.C.C.S., Polytechnic University of the Marche Region, Ancona, Italy cInstitute of Infectious Diseases and Public Health, Polytechnic University of the Marche Region, Ancona, Italy dFaculty of Pharmacy, Medical University of Gdańsk, Poland eExperimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.C.C.S., Ancona, Italy fFaculty of Chemistry, University of Gdańsk, Poland |
| Abstract: | We investigated the efficacy of Tachyplesin III alone or combined with piperacillin-tazobactam (TZP) to prevent biofilm formation in vitro and in a rat model of Pseudomonas aeruginosa ureteral stent infection. We have observed that in vitro TZP, in presence of Tachyplesin III, showed minimal inhibitory concentrations (MIC)s twofold and minimal bactericidal concentrations (MBC)s eightfold lower. The in vivo study showed that rats that received intraperitoneal TZP showed the lowest bacterial numbers. Tachyplesin III combined with TZP showed efficacies higher than that of each single compound. Coating ureteral stents with Tachyplesin III is able to inhibit bacterial growth up to 1000 times. |
| Keywords: | Peptides Tachyplesin III Ureteral stents Bacterial adhesion Urinary tract infections Biofilm |
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