How to build an inexpensive cyclotherm instrument for automated polymerase chain reaction |
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| Authors: | M Collasius H Falk C Ciesler G Valet |
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| Affiliation: | Mildred-Scheel-Labor für Krebszellforschung, Max-Planck-Institut, Martinsried, Federal Republic of Germany. |
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| Abstract: | The Cyclotherm instrument is a functionally fully equivalent but inexpensive alternative to commercial instruments for automated polymerase chain reaction (PCR). It can be rebuilt under conditions of a biochemical laboratory for less than +1000. A Peltier element is used for heating and cooling of the reaction vials and the temperature and timing of the PCR cycles are controlled by a BASIC program in a SHARP PC 1600 low cost computer. |
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