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Stoichiometry of nitrite reduction catalyzed by Pseudomonas aeruginosa nitrite-reductase |
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| Authors: | Philippe Bessières Yann Henry |
| Institution: | Laboratoire de Biophysique, Institut de Biologie Physico-chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France |
| Abstract: | The stoichiometry of the reduction of nitrite catalyzed by Pseudomonas aeruginosa nitrite-reductase (cytochrome cd1) has been shown to yield nitrous oxide as the final product. Gas chromatography experiments demonstrated that nitric oxide is also formed as a free intermediate. A sequential formation of NO and N2O is discussed as proposed to the parallel formation of the two products. |
| Keywords: | Mots-clés nitrate-réductase' oxyde azotique oxyde azoteux nitrite-reductase nitric oxide nitrous oxide PMS Phenazine methosulfate FMN Flavin mononucleotide Flavin adenine dinucleotide reduced form NADH Nicotinamide adenine dinucleotide Nicotinamide adenine dinucleotide oxidized form EPR Electron paramagnetic resonance TMPD N N′ SDS Sodium dodecylsulfate |
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