Correlation of estrogen and androgen receptor status in prostatic disease measured by high pressure liquid chromatography |
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| Authors: | R Y Kirdani E J Pontes G P Murphy A A Sandberg |
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| Institution: | 1. Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;3. Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;4. George M. O''Brien Research Center of Excellence, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;5. Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;2. Cancer Biology Graduate Program, Department of Oncology, University of Wisconsin–Madison, Madison, Wisconsin;1. Department of Urology, University of Wisconsin School of Medicine and Public Health, 1685 Highland Ave., Madison, WI 53705, USA;2. Cancer Biology Graduate Program, University of Wisconsin-Madison, Wisconsin Institute for Medical Research, 1111 Highland Ave., Madison, WI 53705, USA;3. Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, 600 Highland Ave., Madison, WI 53705, USA;4. George M. O’Brien Research Center of Excellence, University of Wisconsin School of Medicine and Public Health, 1685 Highland Ave., Madison, WI 53705, USA;1. Department of Urology, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin;5. Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin;2. Department of Cancer Biology Graduate Research Program, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin;3. George M. O''Brien Research Center of Excellence, University of Wisconsin, Madison, Wisconsin;4. Carbone Cancer Center, University of Wisconsin, Madison, Wisconsin |
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| Abstract: | To further characterize human prostatic estrogen receptors (ER) determined by high pressure liquid chromatography (HPLC), we checked our procedure by which we nullify estrogen binders (including testosterone binding globulin, TeBG) other than ER by preincubation of cytosols with dihydrotestosterone (DHT). We also showed that the ER exhibited ligand specificity and that ER is present in BPH nuclear extract at 10-fold its concentration in the corresponding cytosols. Of seven prostates with localized cancer determined preoperatively, only 3 showed localization; ER concentration in the cancer parts was lower than in the corresponding surrounding BPH. A total of 22 specimens were evaluated for ER and androgen receptors (AR). Statistically, AR had higher values than ER but there was no correlation between the corresponding AR and ER for each tissue. |
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