Spheroid formation and functional restoration of human fetal hepatocytes on poly-amino acid-coated dishes after serial proliferation |
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Authors: | Taku Matsushita Kohji Nakano Yasufumi Nishikura Kenta Higuchi Akifumi Kiyota Ryuichi Ueoka |
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Institution: | (1) Department of Applied Life Science, Faculty of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto, [860-0082, Japan(e-mail |
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Abstract: | Primary human fetal hepatocytes proliferated in monolayer culture up to the 9th passage. During proliferation, the cells changed
their morphology from a fibroblast-like shape after inoculation to an epithelia-like polygonal shape after they reached confluence.
The proliferation was associated with the loss of ammonia detoxification capacity, which is essential for the function of
bioartificial liver. The cells formed spheroids on a poly-glutamic acid- or poly-aspartic acid-coated polystyrene dish that
had a negatively charged surface at neutral pH. However, the cells did not form spheroids on a poly-lysine- or poly-arginine-coated
dish that had a positively charged surface, which is reportedly suitable to form spheroids for adult hepatocytes. The activity
of cytochrome P450 (CYP 1A1, CYP1A2) of the cells in spheroid culture was about twice as high as that of the cells in monolayer
culture. The ammonia detoxification activity of the cells was restored in spheroid culture by treatment with 2% dimethylsulfoxide.
These results suggest that the conditions for human fetal hepatocytes to form spheroids are different from that for adult
hepatocytes, and the use of poly-glutamic acid or poly-aspartic acid coating may improve spheroid culture of proliferative
human fetal hepatocytes.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Ammonia detoxification Bioartificial liver Cytochrome P450 Human fetal hepatocyte Poly-aspartic acid Poly-glutamic acid Spheroid |
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