Recombinant human granulocyte colony-stimulating factor enhances superoxide release in human granulocytes stimulated by the chemotactic peptide |
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| Authors: | S Kitagawa A Yuo L M Souza M Saito Y Miura F Takaku |
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| Institution: | 1. Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, USA;2. Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA;3. Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, the Netherlands;4. Department of Epidemiology, Cardiovascular Health Research Unit, University of Washington, Seattle, WA, USA;5. Department of Geriatric Medicine, University of Hawaii, Honolulu, HI, USA;6. Atlanta VA Medical Center, Decatur, GA, USA;7. Division of Endocrinology and Metabolism, Emory University School of Medicine, Atlanta, GA, USA;8. Department of Neurology, State University of New York Downstate Medical Center, Brooklyn, NY, USA;9. Department of Family Medicine, Alpert Medical School, Brown University, Providence, RI, USA;10. Department of Epidemiology, School of Public Health, Brown University, Providence, RI, USA;11. Program on Prevention Outcomes and Practices, Stanford Prevention Research Center, Stanford University, Palo Alto, CA, USA;1. Division of Gynecologic Oncology (Drs. Bixel and Barrington);2. Division of Surgical Pathology (Dr. Suarez), College of Medicine;3. Division of Epidemiology, College of Public Health (Dr. Felix), The Ohio State University, Columbus, Ohio;4. Division of Gynecologic Oncology, Baptist Health Medicine Group, Lexington, Kentucky (Dr. Vetter);1. Department of Psychology & Human Development, Peabody College, Vanderbilt University, 230 Appleton Place, Nashville, TN 37203, United States of America;2. Division of Psychology, School of Social Sciences, Nanyang Technological University, 48 Nanyang Avenue, Singapore 639818, Singapore |
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| Abstract: | Recombinant human granulocyte colony-stimulating factor (G-CSF) by itself was not an effective stimulus for inducing the release of superoxide (O-2) in human granulocytes. However, G-CSF was able to prime human granulocytes, and enhanced O-2 release stimulated by the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (FMLP). The preincubation with G-CSF for 5-10 min at 37 degrees C was sufficient for priming the cells. The optimal enhancing effect was obtained at 25 ng/ml of G-CSF. The enhancement of O-2 release by G-CSF was observed over the complete range of effective concentrations of FMLP (10(-8)-10(-6) M). These findings indicate that G-CSF is a potent activator of mature granulocyte functions. |
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