| 实时定量PCR技术及其应用 |
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| 引用本文： | 王梁燕,洪奇华,张耀洲.实时定量PCR技术及其应用[J].细胞生物学杂志,2004,26(1):62-67. |
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| 作者姓名： | 王梁燕 洪奇华 张耀洲 |
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| 作者单位： | [1]浙江大学生命科学学院 [2]动物科学学院，杭州310029 |
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| 摘 要： | 实时定量PCR(Real—time Quantitative Polymerase Chain Reaction，RQ—PCR)技术是20世纪90年代中期发展起来的一种新型核酸定量技术。该技术具有实时监测、快速、灵敏、精确等特点，是对原有PCR技术的革新，扩大了PCR的应用范围。本文综述了RQ—PCR技术的原理、RQ—PCR仪、RQ—PCR实时定量检测系统及其应用。
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| 关 键 词： | 实时定量 PCR 聚合酶链式反应 体外扩增 |
| 文章编号： | 0253-9977(2004)01-62-06 |
| 修稿时间： | 2003年5月23日 |
Real-time Quantitative PCR and Its Applications |
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| WANG Liang Yan,HONG Qi Hua,ZHANG Yao Zhou.Real-time Quantitative PCR and Its Applications[J].Chinese Journal of Cell Biology,2004,26(1):62-67. |
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| Authors: | WANG Liang Yan HONG Qi Hua ZHANG Yao Zhou |
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| Abstract: | Developed in the mid 1990s for the analysis and quantification of nucleic acid, realtime quantitative PCR is a molecular biological technique gaining rapidly in popularity. The advancement provided by the real-time version of PCR is due to its unique ability to monitor the complete DNA amplification progress and other features such as rapidness, sensitivity and accuracy. Real-time Quantitative PCR has reformed the conventional PCR as well as enlarged applications. This article reviews the principle, instruments, detection systems for quantification and some applications of this new technique. |
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| Keywords: | real-time quantitative PCR applications |
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