首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Comparison of the sequences of the nagE operons from Klebsiella pneumoniae and Escherichia coli K12: enhanced variability of the enzyme IIN-acetylglucosamine in regions connecting functional domains.
Authors:Alfried P Vogler and Joseph W Lengeler
Institution:(1) Dept. of Molecular Biophysics and Biochemistry, Yale University, P.O. Box 6666, 06511 New Haven, Connecticut, USA;(2) Fachbereich Biologic/Chemie, Universität Osnabrück, Postfach 4469, W-4500 Osnabrück, Germany
Abstract:Summary The nagE operon, encoding the enzyme II specific for N-acetylglucosamine (EIINag), and adjacent DNA from the chromosome of Klebsiella pneumoniae were sequenced and compared with the corresponding sequence from Escherichia colt K12. The deduced EIINag sequences differ in 72 out of 651 amino acids, the K. pneumoniae sequence being three residues longer. The amino acid differences were distributed unevenly, and were most frequent in regions connecting the three functional domains of the protein. In the nagE-nagB intergenic region, two promoter, two operator, and one CAP consensus sequence with regulatory functions were highly conserved. The nag structural genes from both species were very similar (83% DNA similarity; 89% amino acid similarity) except for frequent AT to GC exchanges in the wobble base of codons in K. pneumoniae DNA relative to the E. coli DNA.
Keywords:nagE operon  Enzyme IINag  Functional domains  Linker
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号