Influence of growth rate on the accumulation of ergosterol in yeast-cells in a phosphate limited continuous culture |
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Authors: | C Arnezeder W A Hampel |
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Institution: | (1) Institute of Biochemical Technology and Microbiology, Technical University of Vienna, Getreidemarkt 9, A-1060 Vienna, (Austria) |
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Abstract: | Summary The influence of the growth rate on the accumulation of ergosterol inSaccharomyces cerevisiae was studied with glucose and ethanol as substrates under P-limitation in chemostat experiments. In cultures with glucose as carbon source a decrease in ergosterol content with dilution rates up to 0.08 h–1 was observed, whereas above this dilution rate an increase in ergosterol content occurred. Similar but less marked effects were attained with ethanol as carbon source. A maximum specific rate of ergosterol synthesis of about 2.4 mg per h and g dry cell mass was calculated for phosphorus limited cultures. |
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