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Fluorescence resonance energy transfer (FRET)-based subcellular visualization of pathogen-induced host receptor signaling |
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| Authors: | Alexander Buntru Timo Zimmermann Christof R Hauck |
| Affiliation: | 1. Lehrstuhl für Zellbiologie, Universit?t Konstanz, Konstanz, Germany 2. Konstanz Research School Chemical Biology, Universit?t Konstanz, Konstanz, Germany 3. Advanced Light Microscopy Unit, CRG-Centre de Regulació Genòmica, Barcelona, Spain |
| Abstract: | BackgroundBacteria-triggered signaling events in infected host cells are key elements in shaping the host response to pathogens. Within the eukaryotic cell, signaling complexes are spatially organized. However, the investigation of protein-protein interactions triggered by bacterial infection in the cellular context is technically challenging. Here, we provide a methodological approach to exploit fluorescence resonance energy transfer (FRET) to visualize pathogen-initiated signaling events in human cells. |
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