Identification and cDNA sequence of delta-preprotachykinin, a fourth splicing variant of the rat substance P precursor. |
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| Authors: | A J Harmar V Hyde K Chapman |
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| Institution: | MRC Brain Metabolism Unit, Royal Edinburgh Hospital, Morningside Park, UK. |
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| Abstract: | The neuropeptides substance P and neurokinin A are synthesised from a family of precursor polypeptides encoded by the preprotachykinin A (PPT) gene. In addition to a mRNA (beta-PPT) containing all 7 exons of the gene, alternatively spliced mRNAs lacking either exon 4 (gamma-PPT) or exon 6 (alpha-PPT) have been identified. We have determined the sequences of cDNA clones encoding four variants of PPT mRNA from rat dorsal root ganglion (DRG), including a novel mRNA species (delta-PPT) in which both exons 4 and 6 are absent. The sequence of delta-PPT predicts the existence of a novel tachykinin precursor polypeptide. |
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