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Identification of an allelic variant of isoform MLC1-V/sB (human myosin light chain) |
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| Authors: | Alexei V. Laptev Sergei S. Shishkin Leonid I. Kovalyov Marina A. Galyuk Alexander Kh. Musolyamov Tsezi A. Egorov |
| Affiliation: | (1) Research Centre of Medical Genetics, Russian Academy of Medical Sciences, Moscvorechye str., 1, 115478 Moscow;(2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str., 32, 117984 Moscow |
| Abstract: | A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping afterin situ tryptic digestion of electroblotted proteins. The substitution Asn-144 for His-144 was found in this new allelic variant of MLC1-V/sB.This work was supported by a grant from Russian Human Genome Project. |
| Keywords: | myosin light chain human heart two-dimensional polyacrylamide gel electrophoresis peptide mapping microsequencing |
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