Determination of riboflavin by high-performance liquid chromatography with riboflavin-depleted urine as calibration and control matrix |
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Authors: | Chen Meng Andrenyak David M Moody David E Foltz Rodger L |
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Affiliation: | Center for Human Toxicology, Department of Pharmacology and Toxicology, University of Utah, 20 South 2030 East Room 490, Salt Lake City, UT 84112, USA. mc4@utah.edu |
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Abstract: | A simple method, exposure to natural-light, was developed to remove riboflavin from urine to enhance its use as the biological matrix for the preparation of calibration and control samples. Riboflavin-depleted urine containing less than 1 ng/ml of riboflavin was used to validate a high-performance liquid chromatography with fluorescence detection method for the determination of urinary riboflavin. The linearity of the assay (r2=0.999) was acceptable over the range of 10-5000 ng/ml. The intra-assay and inter-assay CVs were 3.3% and 9%, respectively. Subsequent stability studies found that urine riboflavin was stable for up to 6 months at 4 or -20 degrees C. |
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Keywords: | Riboflavin Urine HPLC Stability |
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