Regeneration of fertile wheat (Triticum aestivum) plants from isolated zygotes using wheat microspore culture as nurse cells |
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Authors: | Bakos F Darkó É Pónya Zs Barnabás B |
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Institution: | (1) Agricultural Research Institute of the Hungarian Academy of Sciences, P.O.B. 19, H-2462 Martonvásár, Hungary |
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Abstract: | A new method for growing isolated wheat zygotes by co-cultivation with microspores isolated from the same species has been developed. Although the mortality of isolated zygotes within 6 h after the transfer into the nurse culture was relatively high (70%) in this method, the majority of the structures which survived developed into fertile plants (61%). Zygotic structures grown in these cultures were morphologically not as regular as embryos grown in planta, but they resembled them closely in cytological properties and developmental pattern. |
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Keywords: | co-cultivation embryo hormone-like compounds in vitro in vivo fertilized zygotes |
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