The Role of Abscisic Acid in Acclimation of Plants Cultivated in vitro to ex vitro Conditions |
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Authors: | M Hronková H Zahradní?ková M ?imková P ?imek A Heydová |
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Institution: | (1) Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic;(2) Institute of Entomology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic;(3) Faculty of Biology, University of South Bohemia, Branišovská 31, CZ- 37005 České Budějovice, Czech Republic |
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Abstract: | The content of endogenous free abscisic acid (ABA) in the shoots of in vitro cultivated tobacco (Nicotiana tabacum L. cv. White Burley) and its changes during ex vitro acclimation of these plants to the greenhouse or growth chamber were estimated. The content of free ABA significantly increased
at the 1st and/or 2nd day after plant transfer from in vitro to ex vitro. The ABA content of plants covered with transparent foil to maintain higher relative humidity (RH), did not significantly
differ from ABA content of plants cultivated under ambient RH. Transfer to fresh medium also transiently increased the content
of endogenous ABA. The ABA content in plants, which had been acclimated for 1 week to ex vitro conditions, decreased to the content found in the in vitro plants. Acclimation to ex vitro conditions affected the stomata on adaxial and abaxial sides differently: stomata on the adaxial side were less open than
those on the abaxial one. The exogenous application of 5 μM ABA increased transiently its endogenous concentration in shoots
of in vitro plants more than ten fold, but after 1 week the concentration in the shoots decreased.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Nicotiana tabacum stomata |
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