Thermal stable and oxidation-resistant variant of subtilisin E |
| |
Authors: | Yang Y Jiang L Zhu L Wu Y Yang S |
| |
Affiliation: | Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, 200233, Shanghai, PR China. yonghuayang@hotmail.com |
| |
Abstract: | A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at position 118. The half-life of M222A/N118S enzyme activity, when heated at 65 degrees C, was approximately 80 min while the half-life of M222A and wild-type subtilisin E were 13 min and 15 min, respectively. This suggested the stability of the M222A/N118S mutant was five times greater than that of the wild-type enzyme. The mutant was also as oxidation resistant as the mutant M222A of subtilisin E. These results indicated the M222A/N118S mutant is both an oxidation-resistant and a heat-stable variant of subtilisin E. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|