Synthetic xylan-binding modules for mapping of pulp fibres and wood sections |
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Authors: | Lada Filonova Lavinia Cicortas Gunnarsson Geoffrey Daniel Mats Ohlin |
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Institution: | (1) Department of Wood Science, WURC, Swedish University of Agricultural Sciences, PO Box 7008, SE-750 07 Uppsala, Sweden;(2) Department of Immunotechnology, Lund University, BMC D13, S-22184 Lund, Sweden;(3) Current address : Affitech AS, Oslo, Norway |
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Abstract: | Background The complex carbohydrate composition of natural and refined plant material is not known in detail but a matter that is of
both basic and applied importance. Qualitative assessment of complex samples like plant and wood tissues requires the availability
of a range of specific probes. Monoclonal antibodies and naturally existing carbohydrate binding modules (CBMs) have been
used in the past to assess the presence of certain carbohydrates in plant tissues. However, the number of natural CBMs is
limited and development of carbohydrate-specific antibodies is not always straightforward. We envisage the use of sets of
very similar proteins specific for defined targets, like those developed by molecular evolution of a single CBM scaffold,
as a suitable strategy to assess carbohydrate composition. An advantage of using synthetic CBMs lies in the possibility to
study fine details of carbohydrate composition within non-uniform substrates like plant cell walls as made possible through
minor differences in CBM specificity of the variety of binders that can be developed by genetic engineering. |
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