Real-time phase-contrast x-ray imaging: a new technique for the study of animal form and function |
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| Authors: | John J Socha Mark W Westneat Jon F Harrison James S Waters and Wah-Keat Lee |
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| Institution: | (1) Advanced Photon Source, Argonne National Laboratory, 9700 S. Cass Ave, Argonne, IL 60439, USA;(2) Department of Zoology, Field Museum of Natural History, 1400 S. Lake Shore Dr, Chicago, IL 60605, USA;(3) Section of Organismal, Integrative, and Systems Biology, School of Life Sciences, Arizona State University, PO Box 874501, Tempe, AZ 85287-4501, USA |
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| Abstract: | Background Despite advances in imaging techniques, real-time visualization of the structure and dynamics of tissues and organs inside
small living animals has remained elusive. Recently, we have been using synchrotron x-rays to visualize the internal anatomy
of millimeter-sized opaque, living animals. This technique takes advantage of partially-coherent x-rays and diffraction to
enable clear visualization of internal soft tissue not viewable via conventional absorption radiography. However, because
higher quality images require greater x-ray fluxes, there exists an inherent tradeoff between image quality and tissue damage. |
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