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Microarray-based method for detection of unknown genetic modifications |
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| Authors: | Torstein Tengs Anja B Kristoffersen Knut G Berdal Tage Thorstensen Melinka A Butenko Håvard Nesvold Arne Holst-Jensen |
| Institution: | (1) National Veterinary Institute, Section of Feed and Food Microbiology, PO Box 8156 Dep, 0033 Oslo, Norway;(2) National Veterinary Institute, Section of Epidemiology, PO Box 8156 Dep, 0033 Oslo, Norway;(3) Department of Informatics, University of Oslo, PO Box 1080, 0316 Blindern, Oslo, Norway;(4) Department of Molecular Biosciences, University of Oslo, PO Box 1041, 0316 Blindern, Oslo, Norway |
| Abstract: | BackgroundDue to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed a strategy for detection and characterization of unknown genetic modifications and we present a proof of concept for this method using Arabidopsis thaliana and Oryza sativa (rice). The approach relies on direct hybridization of total genomic DNA to high density microarrays designed to have probes tiled throughout a set of reference sequences. |
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