| 氨基酸制备色谱-Dpsc离子交换树脂柱分离发酵L-缬氨酸 |
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| 引用本文： | 汤家芳,张一竹.氨基酸制备色谱-Dpsc离子交换树脂柱分离发酵L-缬氨酸[J].氨基酸和生物资源,2005,27(3):43-45. |
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| 作者姓名： | 汤家芳 张一竹 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072;武汉大学生命科学学院,武汉,430072 |
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| 摘 要： | 从苯乙烯、甲基丙烯酸甲酯、二乙烯苯合成了具有磺酸、羧酸功能基团的新型Dpsc离子交换树脂,并对其表征了选择性、分离度、理论等板高度。用新型Dpsc离子交换树脂在制备规模从发酵缬氨酸液中分离制备了L-缬氨酸,应用0.2mo·L-1NH4Cl和0.5mol·L-1NH4OH,流速6.0mL·min-1,温度25℃,梯度洗脱,减少保留体积4/5。
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| 关 键 词： | 氨基酸 离子交换树脂 制备色谱 发酵缬氨酸 梯度洗脱 |
| 文章编号： | 1006-8376-0043-03 |
| 收稿时间： | 05 18 2005 12:00AM |
| 修稿时间： | 2005年5月18日 |
Amino Acid - Preparative Chromatography--Separation of L - Valine Fermented Broth on Dpsc Ion- Exchange Resin Column |
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| TANG Jia-fang,ZHANG Yi-zhu.Amino Acid - Preparative Chromatography--Separation of L - Valine Fermented Broth on Dpsc Ion- Exchange Resin Column[J].Amino Acids & Biotic Resources,2005,27(3):43-45. |
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| Authors: | TANG Jia-fang ZHANG Yi-zhu |
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| Abstract: | A new Dpsc ion-exchange resin was synthesized with sulforic acid and carboxylic acid from styrene-methyl methylacrylic acid-divinyl benzene(vinyl styrene). The new Dpsc ion-exchange resin was characterized with the selectivity factor, resolution and The height equivalent to a theoretical plate. The L-valine was prepared with Dpsc ion-exchange resin from valine fermented broth on a preparation scale. The retention volume was reduced to four fifth by 0.2moL/L and 0.5moL/L gradient elution on the new Dpsc ion-exchange resin column at a flow rate of 6ml/min at 25℃. |
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| Keywords: | amino acids ion - exchange resin preparative chromatography L - valine - fermented broth gradient elution |
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