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pH控制对热凝胶发酵的影响
引用本文:王磊 詹晓北 朱一晖 李珍雨 杨烨. pH控制对热凝胶发酵的影响[J]. 生物工程学报, 2002, 18(5): 634-637
作者姓名:王磊 詹晓北 朱一晖 李珍雨 杨烨
作者单位:1. 江南大学工业生物技术教育部重点实验室,无锡,214036
2. Diversion of Biotechnology,Donga University,Pusan 604-714,Korea
基金项目:江苏省科技攻关 (国际合作 )项目基金资助 (No .BS99719)~~
摘    要:热凝胶 (Curdlan)是一种直链结构的 β 1,3 葡聚糖 ,由Alcaligenesfaecalisvar.myxogenes发酵生产而来 ,是一种新型的微生物胞外多糖[1 ] ,其分子量在 5 0万左右。热凝胶在中性条件下不溶于水 ,但能溶于碱溶液中。加热含有热凝胶的水浊液可形成两种类型的凝胶 ,一种是弹性较低的类似琼脂的可逆胶 ;另外一种是凝胶强度大、弹性好的热不可逆胶。由于热凝胶具有独特的热成胶性能 ,在食品工业 ,特别是高温制作的食品领域具有广阔的应用前景。热凝胶的胶体可以包容和控制药物的扩散 ,所以可以用来作为药物…

关 键 词:产碱杆菌,热凝胶发酵,pH控制,两阶段
文章编号:1000-3061(2002)05-0634-04
修稿时间:2002-04-15

Influence of pH Control on the Production of Curdlan by Alcaligenes faecalis Strain
WANG Lei ZHAN Xiao-Bei ZHU Yi-Hui LI Zhen-Yu YANG Ye. Influence of pH Control on the Production of Curdlan by Alcaligenes faecalis Strain[J]. Chinese journal of biotechnology, 2002, 18(5): 634-637
Authors:WANG Lei ZHAN Xiao-Bei ZHU Yi-Hui LI Zhen-Yu YANG Ye
Affiliation:Key Laboratory of Industrial Biotechnology of Ministry of Education, Southern Yangtze University, Wuxi 214036, China.
Abstract:A two-stage pH control method was employed in batch fermentation of curdlan by Alcaligenes faecalis WX-C12 where cell-growth stage was constantly controlled at pH 7.0 and stationary stage was controlled at a constant pH as well. The influence of pH control on the curdlan production was investigated. The optimal pH control of batch process for curdlan production was obtained when cell-growth stage was controlled at pH 7.0 and stationary stage was constantly controlled at pH 5.6. Production and productivity of curdlan, QP and YP/S reached 28.19 g/L, 291 mg/(L.h), 132.27 mg/(L.h.g) and 0.659, an improvement of 20.4%, 38.1%, 38.1% and 29.5% compared to a pH uncontrolled operation respectively.
Keywords:Alcaligenes faecalis   curdlan fermentation   pH control   two-stage
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