Identification of a human platelet membrane protein alkylated under conditions inhibitory of phospholipase A2 activity |
| |
Authors: | S T Silk M E DeMarco |
| |
Institution: | Lab. Physiol. Fac. Med. Grange-Blanche, Lyon, France. |
| |
Abstract: | Platelet membrane sulfhydryls essential for phospholipase A2 activity were alkylated by 3H]N-ethylmaleimide after the non-essential sulfhydryls were cross-linked by azodicarboxylic acid bis(di-methylamide) (DA) or alkylated by N-ethylmaleimide. A 24.5K da protein labeled under phospholipase inhibitory conditions was not labeled under non-inhibitory conditions. The polypeptide, which had neither endogenous nor DA induced disulfides, may be a platelet membrane phospholipase A2 or a lipase regulatory protein. |
| |
Keywords: | |
|
|