| Val 70,Phe 72 and the last seven amino acid residues of C—terminal are essential to the function of norepinephrine transporter |
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| 引用本文： | LIUYANHONG WOLFGANGSCHWARZ. Val 70,Phe 72 and the last seven amino acid residues of C—terminal are essential to the function of norepinephrine transporter[J]. Cell research, 1998, 8(4): 311-315 |
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| 作者姓名： | LIUYANHONG WOLFGANGSCHWARZ |
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| 作者单位： | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031,China [2]Max－PlankInstituteforBio,ChineseAcademyofSciences,Shanghai200031,China |
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| 摘 要： | The norepinephrine transporter(NET) is a member of the Na^ /Cl^- dependent neurotransmitter transporter family and constitutes the target of several clinically important antidepressants.To delineate the critical amino acid residues and the function of C-terminal in regulating transport activity of NET,here we constructed two site mutants (V70F,F72V;V70I,F72V) and one C-terminal truncated mutant (Δ 611-617).The wild type and mutants of NET were expressed in Xenopus oocytes by injection of their cRNA.We found that all of these mutants lost their transport activity.These results indicate that the amino acid residues of V70 and F72,and the last seven amino acids of C-terminal are essential to the transport activity of NET.
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| 关 键 词： | 去甲肾上腺素 神经递质 功能 C端 氨基酸残基 Val 70 Phe 72 |
Val 70, Phe 72 and the last seven amino acid residues of C-terminal are essential to the function of norepinephrine transporter |
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| LIU YAN HONG, FANG HUANG, JIAN FEI,JIAN XING ZHAO, QUANG BAO GU, WOLFGANG SCHWARZ, LI HE GUO. Val 70, Phe 72 and the last seven amino acid residues of C-terminal are essential to the function of norepinephrine transporter[J]. Cell research, 1998, 8(4): 311-315 |
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| Authors: | LIU YAN HONG FANG HUANG JIAN FEI JIAN XING ZHAO QUANG BAO GU WOLFGANG SCHWARZ LI HE GUO |
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| Abstract: | |
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| Keywords: | Norepinephrine transporter neurotransmitter uptake mutagenesis |
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