Low-temperature spectroscopy of isolated FMO-protein and a membrane-free reaction center complex from the green sulfur bacteriumChlorobium tepidum |
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Authors: | Mette Miller Raymond P. Cox John M. Olson |
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Affiliation: | (1) Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark |
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Abstract: | We have isolated the water-soluble BChla-protein (FMO-protein) from the greer sulfur bacteriumChlorobium tepidum by a new procedure involving a salt-wash of isolated membranes at alkaline pH. The absorption spectrum of the isolated FMO-protein at 77 K was compared with that of a reaction-center complex containing the FMO-protein (FMO-RC complex) isolated fromC. tepidum following the procedure of Feiler U, Nitsche W and Michel H (1992) Biochemistry 31: 2608–2614. Oxidation or illumination of the FMO-RC complex caused bleaching of a component with a maximum at 836 nm which was not present in the purified FMO-protein. |
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Keywords: | BChla-protein green sulfur bacteria reaction center complex Chlorobium tepidum |
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