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Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
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| Authors: | Claes Filip Radwanska Magda Urakawa Toyo Majiwa Phelix Ao Goddeeris Bruno Büscher Philip |
| Institution: | (1) Department of Animal Science, Faculty of Agriculture and Applied Biological Sciences, K. U. Leuven, Kasteelpark Arenberg 30, 3000 Leuven, Belgium;(2) Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, Antwerpen, Belgium;(3) International Livestock Research Institute (ILRI), Nairobi, Kenya |
| Abstract: | BackgroundBased on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). |
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