Synthesis of non-natural DNA using DNA polymerase. |
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| Authors: | Y Ebara T Sugiyama K Kaihatsu S Ueji |
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| Affiliation: | Division of Natural Environment and Bioorganic Chemistry, Faculty of Human Development and Sciences, Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe 657-8501, Japan. |
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| Abstract: | Nonnatural nucleotide modified by glucose or galactose was synthesized to increase functional diversity of DNA library. These compounds were incorporated in a DNA double strand using Klenow Fragment as well as dTTP. These functional group could be ordered sequentially on a DNA double strand at intervals of few angstroms according to the designed template sequence within a few hours. This method must be useful to constructing nonnatural DNA library or designed supramolecular structures. |
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