| 抗真菌肽CGA-N46基因多顺反子表达载体的构建与鉴定 |
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| 引用本文： | 李瑞芳,熊前程,张宗武,黄亮,王彬. 抗真菌肽CGA-N46基因多顺反子表达载体的构建与鉴定[J]. 中国生物工程杂志, 2011, 31(12): 93-98 |
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| 作者姓名： | 李瑞芳 熊前程 张宗武 黄亮 王彬 |
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| 作者单位： | 河南工业大学生物工程学院 郑州 450001 |
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| 基金项目： | 国家自然科学基金(31071922); 河南省重点科技攻关项目(0623030300,112102310325)资助项目 |
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| 摘 要： | 目的:为提高抗真菌肽CGA-N46表达量,对该基因多顺反子表达进行研究.方法:以pEASY-Blunt为克隆栽体,以“pET-30a rbs序列-起始密码子-CGA-N46编码序列-终止密码子”为外源片段,利用同尾酶Nhe I、Spe I和Xba I,构建了含有上述外源片段1、3、5、8拷贝的重组载体pT-CAN46、...
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| 关 键 词： | 抗真菌肽 CGA-N46 多肽基因工程表达 多顺反子表达 载体构建 |
| 收稿时间： | 2011-08-06 |
| 修稿时间： | 2011-10-24 |
Construction and Identification of Polycistronic Expression Plasmids of Antifungal Peptide CGA-N46 Gene |
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| LI Rui-fang,XIONG Qian-cheng,ZHANG Zong-wu,HUANG Liang,WANG Bin. Construction and Identification of Polycistronic Expression Plasmids of Antifungal Peptide CGA-N46 Gene[J]. China Biotechnology, 2011, 31(12): 93-98 |
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| Authors: | LI Rui-fang XIONG Qian-cheng ZHANG Zong-wu HUANG Liang WANG Bin |
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| Affiliation: | LI Rui-fang XIONG Qian-cheng ZHANG Zong-wu HUANG Liang WANG Bin (College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China) |
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| Abstract: | Objective: To improve the expression of antifungal peptide CGA-N46,the polycistronic expression of CGA-N46 were studied.Methods: The recombinant cloning plasmids pT-CAN46,pT-3CAN46,pT-5CAN46 and pT-8CAN46 were constructed firstly,in which one copy,three copies,five copies and eight copies of heterogeneous gene fragment which was pET-30a rbs sequence-initiation codon-CGA-N46 encoding sequence-termination codon was recombined with cloning plasmid pEASY-Blunt respectively.And then the recombinant expression pl... |
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| Keywords: | Antifungal peptide CGA-N46 Gene engineering expression of peptide Polycistronic expression Plasmid construction |
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